We converted our 2 horse big trailer into a mini trailer by adding dividers. I basically took our biggest horse and put him in our cart shafts. He had room on both sides. Then I measured the width of the shafts and used that as a starting point for comfort. Our dividers are that wide and about 42 inches tall at the head portion and slope back to 36 inches at the tail portion. This has worked well for us. We took the entire length of the inside of our trailer, front to back, divided it with the shaft width ( we got nearly 5 stall widths). We then set it up so there are 3 individual stall widths and one larger one for mare/foal combo to travel - giving us 4 stalls with 3 dividers.
Since we do not want our horses to have too much room to get into trouble when trailering, we find this works well. None are rubbed on by the dividers, but they can't get into much trouble either. The higher divider (made of metal mesh) at the head allows them to see each other, but not bite. Tail height at the back prevents kicking the other horses. We have hauled a stallion, 2 mares and a mare/foal combo all together with no problems.
I hope this information can give you an idea of where to start to measure. Of course, our trailer is human height-7', which is wonderful in loading.